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Posted by s under Uncategorized. com stats helper monkeys prepared a 2013 annual report for this blog. A San Francisco cable car holds 60 people. This blog was viewed about 1,500. If it were a cable car, it would take about 25 trips to carry that many people. Click here to see the complete report. Posted by s under Chapter 10.
3 place the tubes on ice. 4 put a single colony of bacteria into each tube. 5 incubate the tubes for 10 minutes on ice. 8 pipet 100 µl of the solutions onto the appropriate plates.
In Biology class today, we ran a lab concerning deoxyribonucleic acid, better known as DNA. The pGLO plasmid did .
Posted May 6, 2012 by richardqiu. In this lab we transformed the DNA of E. coli, keeping in mind the flow of genetic information from DNA to RNA to proteins to expressed traits.
From this, we can draw the conclusion that arabinose is mandatory for a GFP. Enjoy! DNA of Wheat Germ.
All about Biology Honors and how NEAT it is! May 7, 2012. In conclusion, we had a successful lab. Two of our plates were also ampicillin resistant so we could say that because we compared them to our control plates. For more info click here. For Wikipedias take on Dinoflagellates click here.
Our latest science lab involved genetically transforming E. coli by using plasmid DNA containing the gene called pGLO. pGLO enables the bacteria to be resistant to ampicillin. It also causes the bacteria to glow under UV light when exposed to arabinose, a sugar that turns on the gene. PGLO, amp, LB. PGLO, amp, ara, LB. PGLO, amp, LB. Above is a picture of the glowing E.
This dish contained bacteria transformed with pGLO. They are in a dish with arabinose, allowing them to fluoresce, and ampicillin which they are resistant to because they are growing. In the photo we are shining a UV light on them to see the florescence. This is solely as a comparison showing that it is difficult to tell that the bacteria are fluorescing without additional UV light. On May 6, 2012. Radio That Makes Your Skin Crawl.
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The site had the following in the homepage, "DNA is deoxyribonucleic acid an long macromolecule that is the main component of chromosomes and is the material that transfers genetic characteristics in all life forms." I noticed that the web site stated " DNA is a double helix of purine." They also stated " While studying cancer, we were asked to created an infographic that displayed key biological concepts in regards to a cancer related topic of study. As I proceeded to learn about the tanning bed and how it contributes to the number of case."ANALYZE MORE BUSINESSES
This dish contained bacteria transformed with pGLO. They are in a dish with arabinose, allowing them to fluoresce, and ampicillin which they are resistant to because they are growing. In the photo we are shining a UV light on them to see the florescence. This is solely as a comparison showing that it is difficult to tell that the bacteria are fluorescing without additional UV light. On May 6, 2012. Radio That Makes Your Skin Crawl.
Wednesday, April 4th, 2012. Thursday, February 16th, 2012. Saturday, February 11th, 2012. org account to have viewing privileges.
E Coli and pGlo Lab. PGlo arabinose under UV light. In this lab we attempted to change the DNA of a weak strain of E Coli using a circular plasmid called pGLO. With the pGLO we wanted to see the E Coli glow when exposed to UV light, as well as being resistant to an antibiotic called ampicillin. The pGLO succeeded in making the E Coli resistant to ampicillin but the E Coli only glowed when in contact with arabinose.
In this experiment we inserted plasmid DNA into non-disease-causing E. coli bacteria, making them glow and antibiotic resistant. We did this to observe the connections between DNA to RNA to protein to a trait. We used GFP as a biological tracer to see if we had transformed the. Cells to accept the pGLO plasmid DNA.
Extracting DNA from Wheat Germ. Today, we extracted DNA from wheat germ. Background Information on Crystal Jelly-Also known as Aequorea victoria, the crystal jelly is found of the coast of North America. Crystal jelly contains a protein called.