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3 place the tubes on ice. 4 put a single colony of bacteria into each tube. 5 incubate the tubes for 10 minutes on ice. 8 pipet 100 µl of the solutions onto the appropriate plates.
Posted May 6, 2012 by richardqiu. In this lab we transformed the DNA of E. coli, keeping in mind the flow of genetic information from DNA to RNA to proteins to expressed traits.
From this, we can draw the conclusion that arabinose is mandatory for a GFP. Enjoy! DNA of Wheat Germ.
All about Biology Honors and how NEAT it is! May 7, 2012. In conclusion, we had a successful lab. Two of our plates were also ampicillin resistant so we could say that because we compared them to our control plates. For more info click here. For Wikipedias take on Dinoflagellates click here.
Our latest science lab involved genetically transforming E. coli by using plasmid DNA containing the gene called pGLO. pGLO enables the bacteria to be resistant to ampicillin. It also causes the bacteria to glow under UV light when exposed to arabinose, a sugar that turns on the gene. PGLO, amp, LB. PGLO, amp, ara, LB. PGLO, amp, LB. Above is a picture of the glowing E.
These photos are all of our bacteria plates.
DNA is a double helix of purine. While studying cancer, we were asked to created an infographic that displayed key biological concepts in regards to a cancer related topic of study. As I proceeded to learn about the tanning bed and how it contributes to the number of case.
This dish contained bacteria transformed with pGLO. They are in a dish with arabinose, allowing them to fluoresce, and ampicillin which they are resistant to because they are growing. In the photo we are shining a UV light on them to see the florescence. This is solely as a comparison showing that it is difficult to tell that the bacteria are fluorescing without additional UV light. On May 6, 2012. Radio That Makes Your Skin Crawl.
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Here it is, March already, and I feel as though I just walked through the door. Time is flying and I am walking, trying to catch up in all aspects of my life, both personal and professional.
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