3 place the tubes on ice. 4 put a single colony of bacteria into each tube. 5 incubate the tubes for 10 minutes on ice. 8 pipet 100 µl of the solutions onto the appropriate plates.

Posted May 6, 2012 by richardqiu. In this lab we transformed the DNA of E. coli, keeping in mind the flow of genetic information from DNA to RNA to proteins to expressed traits.

This dish contained bacteria transformed with pGLO. They are in a dish with arabinose, allowing them to fluoresce, and ampicillin which they are resistant to because they are growing. In the photo we are shining a UV light on them to see the florescence. This is solely as a comparison showing that it is difficult to tell that the bacteria are fluorescing without additional UV light. On May 6, 2012. Radio That Makes Your Skin Crawl.

Below are two pictures of the samples containing pGLO DNA, LB, ampicillin, and arabinose under UV light. The Process of DNA Extraction. In this experiment, we extracted DNA from wheat germ. The DNA was white, cloudy, and thick. Kendra found this very interesting. The Effect of Color on Photosynthesis.

Discovering how neat nature really is! E Coli Transformation lab. On May 6, 2012. PGLO plate with only LB. PGLO with LB, amp, and arabinose. PGLO with LB and amp. PGLO with LB and amp. The informative infographic of information. On April 5, 2012. Please enjoy this lovely infographic concerning stats about cancer in the US of A! On February 16, 2012.

Potted Plants at the End of the Rainbow. Be sure to check out the videos at the bottom of the Procedure page! November 30, 2011.