In Biology class today, we ran a lab concerning deoxyribonucleic acid, better known as DNA. The pGLO plasmid did .

This dish contained bacteria transformed with pGLO. They are in a dish with arabinose, allowing them to fluoresce, and ampicillin which they are resistant to because they are growing. In the photo we are shining a UV light on them to see the florescence. This is solely as a comparison showing that it is difficult to tell that the bacteria are fluorescing without additional UV light. On May 6, 2012. Radio That Makes Your Skin Crawl.

Once the plasmid was inserted into the bacteria, it was able to fluoresce. These are the four different dishes of bacteria.

This photo shows that under UV light, the only colonies that fluoresced were the pGLO which had arabinose in their environment.

Thanks for dropping by samcareybiology! Take a look around and grab the RSS feed. Mdash; Leave a comment. Plate with -pGLO and just LB. In this lab, we attempted to complete a transformation of a gene into a bacteria. We used plasmids, small rings of DNA, to insert a new gene into.

Where it is copied into a form of RNA by the RNA polymerase. This process goes through 3 stages starting with. Where the RNA polymerase binds to the DNA on a certain location. Where the copying is complete and the RNA can then proceed.

On May 6, 2012. On April 5, 2012. On February 17, 2012. N order to seperate the DNA, we had to first dissolve the cell membrane. Once the detergent had dissolved the nuclear and cellular membranes, the DNA was released. Because the DNA is less dense than water, it floats above the water. In alcohol, DNA forms into clusters and strands, which is what we could see in our test tube. On February 10, 2012. On November 29, 2011. On November 16, 2011.

In this experiment we tried to modify E. coli bacteria with GFP, a gene that should allow the bacteria to both fluoresce, something done when arabinose, a sugar, was added to their nutrients. This was done by injecting the bacteria with a plasmid called pGLO, a plasmid that had both the gene for GFP as well as a gene that would code for a resistance to ampicillin.

While we did not make our own pictures of our lab results this picture shows the identical results. The connection between geography and cancer.

3 We found that adding the pGLO DNA to the bacteria made them ampicillin resistant. My infographic is about the relationship between the GDP per capita of a country and the number of deaths in a country.